Fig. 4From: Metallothionein ameliorates burn sepsis partly via activation of Akt signaling pathway in mice: a randomized animal studyIL-1β (a) and IL-6 (b) mRNA expression in cell lysates were detected by quantitative real-time PCR. Data were calculated using the 2-ΔΔCt method, where Ct is cycle threshold. c The protein levels of pAkt and Akt were determined by western blot. Data were presented as means ± SD. Results are representative of at least three independent experiments. *p < 0.01Back to article page