Fig. 1
From: Evaluation of capillary leakage after vasopressin resuscitation in a hemorrhagic shock model
Timeline experiment. Forty-five rats underwent anesthesia and artery cannulation. Forty rats were subjected to pressure-controlled hemorrhage over a 10-min period, while five rats were sham-operated. The target value of hemorrhagic shock was a reduction in mean arterial pressure (MAP) to 35–40 mmHg. The constant MAP was maintained for 60 min (shock period). After 1 h of shock, 30 hemorrhaged rats were resuscitated over a 10-min period according to the experimental protocol. One hour after the end of resuscitation (observation period), Evans blue dye was injected. Twenty minutes after Evans blue injection, all the rats were euthanized